Testing Foundations of Biological Scaling Theory Using Automated Measurements of Vascular Networks

Scientists have long sought to understand how vascular networks supply blood and oxygen to cells throughout the body. Recent work focuses on principles that constrain how vessel size changes through branching generations from the aorta to capillaries and uses scaling exponents to quantify these changes. Prominent scaling theories predict that combinations of these exponents explain how metabolic, growth, and other biological rates vary with body size. Nevertheless, direct measurements of individual vessel segments have been limited because existing techniques for measuring vasculature are invasive, time consuming, and technically difficult. We developed software that extracts the length, radius, and connectivity of in vivo vessels from contrast-enhanced 3D Magnetic Resonance Angiography. Using data from 20 human subjects, we calculated scaling exponents by four methods--two derived from local properties of branching junctions and two from whole-network properties. Although these methods are often used interchangeably in the literature, we do not find general agreement between these methods, particularly for vessel lengths. Measurements for length of vessels also diverge from theoretical values, but those for radius show stronger agreement. Our results demonstrate that vascular network models cannot ignore certain complexities of real vascular systems and indicate the need to discover new principles regarding vessel lengths

IRAS observations of active galaxies

The IRAS survey gives an unbiased view of the infrared properties of the active galaxies. Seyfert galaxies occupy much the same area in color-color plots as to normal infrared bright galaxies, but extend the range towards flatter 60 to 25 mm slopes. Statistically the Seyfert 1 galaxies can be distinguished from the Seyfert 2 galaxies, lying predominantly closer to the area with constant slopes between 25 and 200 mm. The infrared measurements of the Seyfert galaxies cannot distinguish between the emission mechanisms in these objects although they agree with the currently popular ideas; they do provide a measure of the total luminosity of the Seyferts. The quasar's position in the color-color diagrams continue the trend of the Seyferts. The quasar 3C48 is shown to be exceptional among the radio loud quasars in that it has a high infrared luminosity which dominates the power output of the quasar and is most likely associated with the underlying host galaxy

One Millimeter Continuum Observations of High Redshift Quasars

Upper limits to the one-millimeter continuum flux densities of the high redshift quasars 82 1225 + 31, Ton 490, and PHL 957 are presented. The upper limit to the power observed from these quasars at I mm is, on average, ½ the observed power in the continuum at Lɑ. These observations are used to constrain the temperature of a hypothètical dust shell which reddens the quasar line and continuum emission by an extinction optical depth sufficient to account for the anomalously low Lɑ/Hɑ emission line ratio observed in each of these quasars. For the quasars studied, dust shell temperatures between 25 K and 50 to 95 K are prohibited by the present data. A dust shell at a temperature within this span reradiating all the power absorbed from the quasar ultraviolet continuum would produce a one-millimeter flux density greater than the measured upper limit. The average radius of the model dust shell cannot be between 70 kpc and 1 Mpc

The Behavior of Granular Materials under Cyclic Shear

The design and development of a parallel plate shear cell for the study of large scale shear flows in granular materials is presented. The parallel plate geometry allows for shear studies without the effects of curvature found in the more common Couette experiments. A system of independently movable slats creates a well with side walls that deform in response to the motions of grains within the pack. This allows for true parallel plate shear with minimal interference from the containing geometry. The motions of the side walls also allow for a direct measurement of the velocity profile across the granular pack. Results are presented for applying this system to the study of transients in granular shear and for shear-induced crystallization. Initial shear profiles are found to vary from packing to packing, ranging from a linear profile across the entire system to an exponential decay with a width of approximately 6 bead diameters. As the system is sheared, the velocity profile becomes much sharper, resembling an exponential decay with a width of roughly 3 bead diameters. Further shearing produces velocity profiles which can no longer be fit to an exponential decay, but are better represented as a Gaussian decay or error function profile. Cyclic shear is found to produce large scale ordering of the granular pack, which has a profound impact on the shear profile. There exist periods of time in which there is slipping between layers as well as periods of time in which the layered particles lock together resulting in very little relative motion.Comment: 10 pages including 12 figure

The detection of the J = 3-2 lines of HCN, HNC, and HCO^+ in the Orion molecular cloud

We report the first measurements of the 1.1 mm (J = 3-2) lines of HCN, HNC, and HCO^+ in the Orion molecular cloud. The low-intensity broad velocity wings seen in the (1-0) lines of HCN and HCO^+ are greatly enhanced in the HCN (3-2) line but not in HCO^+ (3-2). No broad wings are seen in the HNC (3-2) line. The HCN observations suggest molecular hydrogen densities ~ 10^6 cm^(-3) in the broad wing source, and the differences between the lines of HCN and HCO^+ suggest that the lines may be formed in different regions within the source

Weight Management Program for Fire Fighters: Feasibility Pilot

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The peritoneal tumour microenvironment of high-grade serous ovarian cancer

High-grade serous ovarian cancer (HGSC) disseminates early and extensively throughout the peritoneal space, causing multiple lesions that are a major clinical problem. The aim of this study was to investigate the cellular composition of peritoneal tumour deposits in patient biopsies and their evolution in mouse models using immunohistochemistry, intravital microscopy, confocal microscopy, and 3D modelling. Tumour deposits from the omentum of HGSC patients contained a prominent leukocyte infiltrate of CD3(+) T cells and CD68(+) macrophages, with occasional neutrophils. Alpha-smooth muscle actin(+) (α-SMA(+) ) pericytes and/or fibroblasts surrounded these well-vascularized tumour deposits. Using the murine bowel mesentery as an accessible mouse peritoneal tissue that could be easily imaged, and two different transplantable models, we found multiple microscopic tumour deposits after i.p. injection of malignant cells. Attachment to the peritoneal surface was rapid (6-48 h) with an extensive CD45(+) leukocyte infiltrate visible by 48 h. This infiltrate persisted until end point and in the syngeneic murine ID8 model, it primarily consisted of CD3(+) T lymphocytes and CD68(+) macrophages with α-SMA(+) cells also involved from the earliest stages. A majority of tumour deposits developed above existing mesenteric blood vessels, but in avascular spaces new blood vessels tracked towards the tumour deposits by 2-3 weeks in the IGROV-1 xenografts and 6 weeks in the ID8 syngeneic model; a vigorous convoluted blood supply was established by end point. Inhibition of tumour cell cytokine production by stable expression of shRNA to CXCR4 in IGROV-1 cells did not influence the attachment of cells to the mesentery but delayed neovascularization and reduced tumour deposit size. We conclude that the multiple peritoneal tumour deposits found in HGSC patients can be modelled in the mouse. The techniques described here may be useful for assessing treatments that target the disseminated stage of this disease

Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater

Chlorinated aliphatic hydrocarbons (CAHs) such as vinyl chloride (VC) and 1,-2 dichioroethane (DCA), are wide-spread groundwater pollutants found at many contaminated field sites around the world. Quantitative tools are needed to determine the in situ rates of VC and DCA transformation to ethene in contaminated groundwater. The objective of this study was to evaluate the potential of E-/Z-chlorofluoroethene (E-/Z-CFE) and DCA-d₄ as surrogates for VC and DCA, respectively. Laboratory microcosm and batch reactors experiments were performed to determine the kinetics and products of VC and E-/Z-CFE transformation to ethene and fluoroethene (FE), respectively. In addition, the products and pathway of DCA and DCA-d₄ also were determined. In all microcosms and batch reactors, E-CFE was preferentially transformed over Z-CFE. For the three experimental systems, the rate of E-CFE transformation to FE was within a factor of 2.7 of that for VC to ethene, which indicates that E-CFE is suitable for use as a surrogate for VC. CFE was later used in a separate field study as a surrogate to model in situ rates of VC reductive dechlorination. The rates of DCA and DCA-d₄ were statistically similar according to a t-test at the 95% confidence level, which also indicates that DCA-d₄ is an excellent surrogate for DCA. Based on this conclusion, DCA-d₄ is now ready to be used in a field test as a surrogate for DCA. This work establishes the precedence for the use of CFE and DCA-d₄ as surrogates for VC and DCA, respectively

Experienced tutors' deployment of thinking skills and what might be entailed in enhancing such skills

In the context of research that reports weaknesses in adults' critical thinking skills, the primary aim was to examine adults' use of critical thinking skills that are described in taxonomies and to identify areas for development. Position papers written by an opportunity sample of 32 experienced adult educators formed the data for a descriptive sample survey design intended to reveal participants' use of critical thinking skills. Each 6000-word paper was written during a development programme that supported such skills. A content analysis of the papers revealed that when participants drew on personal and published ideas about learning to derive their proposals for change, they accepted the ideas uncritically, thereby implying that they might find it difficult to help learners to examine ideas critically. The evidence supports research that implies that critical thinking skills are unlikely to develop unless overall course design privileges the development of epistemological understanding (King and Kitchener 1994, Kuhn 1999). A fundamental assumption underlying the study is that this understanding influences effective citizenship and personal development, as well as employability. A proposition that merits attention in future research is that the development of epistemological understanding is largely neglected in current curricula in formal post-16 education

Randomised feasibility trial into the effects of low-frequency electrical muscle stimulation in advanced heart failure patients.

OBJECTIVES: Low-frequency electrical muscle stimulation (LF-EMS) may have the potential to reduce breathlessness and increase exercise capacity in the chronic heart failure population who struggle to adhere to conventional exercise. The study's aim was to establish if a randomised controlled trial of LF-EMS was feasible. DESIGN AND SETTING: Double blind (participants, outcome assessors), randomised study in a secondary care outpatient cardiac rehabilitation programme. PARTICIPANTS: Patients with severe heart failure (New York Heart Association class III-IV) having left ventricular ejection fraction <40% documented by echocardiography were eligible. INTERVENTIONS: Participants were randomised (remotely by computer) to 8 weeks (5×60 mins per week) of either LF-EMS intervention (4 Hz, continuous, n=30) or sham placebo (skin level stimulation only, n=30) of the quadriceps and hamstrings muscles. Participants used the LF-EMS straps at home and were supervised weekly OUTCOME MEASURES: Recruitment, adherence and tolerability to the intervention were measured during the trial as well as physiological outcomes (primary outcome: 6 min walk, secondary outcomes: quadriceps strength, quality of life and physical activity). RESULTS: Sixty of 171 eligible participants (35.08%) were recruited to the trial. 12 (20%) of the 60 patients (4 LF-EMS and 8 sham) withdrew. Forty-one patients (68.3%), adhered to the protocol for at least 70% of the sessions. The physiological measures indicated no significant differences between groups in 6 min walk distance(p=0.13) and quality of life (p=0.55) although both outcomes improved more with LF-EMS. CONCLUSION: Patients with severe heart failure can be recruited to and tolerate LF-EMS studies. A larger randomised controlled trial (RCT) in the advanced heart failure population is technically feasible, although adherence to follow-up would be challenging. The preliminary improvements in exercise capacity and quality of life were minimal and this should be considered if planning a larger trial. TRIAL REGISTRATION NUMBER: ISRCTN16749049